Sampling strategies for genotyping common bean (Phaseolus vulgaris L.) Genebank accessions with DArTseq: a comparison of single plants, multiple plants, and DNA pools.

ABSTRACT:

Introduction

Genotyping large-scale gene bank collections requires an appropriate sampling strategy to represent the diversity within and between accessions.

Methods

A panel of 44 common bean (Phaseolus vulgaris L.) landraces from the Alliance Bioversity and The Alliance of Bioversity International and the International Center for Tropical Agriculture (CIAT) gene bank was genotyped with DArTseq using three sampling strategies: a single plant per accession, 25 individual plants per accession jointly analyzed after genotyping (in silico-pool), and by pooling tissue from 25 individual plants per accession (seq-pool). Sampling strategies were compared to assess the technical aspects of the samples, the marker information content, and the genetic composition

SUBMITTER: Correa Abondano M 

PROVIDER: S-EPMC11269218 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature