RNA-Seq analysis of the fruiting bodies and mycelia of angel-wing mushroom Pleurocybella porrigens that cause acute encephalopathy.

ABSTRACT:

Objective

In 2004, after consuming angel-wing mushrooms, Pleurocybella porrigens, 59 incidents of food poisoning were reported in Japan. Consequently, 17 individuals died of acute encephalopathy. In 2023, we proved that a lectin, pleurocybelline, and pleurocybellaziridine from this mushroom caused damage to the brains of mice. Although we reported genomic and transcriptomic data of P. porrigens in 2013, the assembly quality of the transcriptomic data was inadequate for accurate functional annotation. Thus, we obtained detailed transcriptomic data on the fruiting bodies and mycelia of this mushroom using Illumina NovaSeq 6000.

Results

De novo assembly data indicated that the N50 lengths for the fruiting bodies and mycelia were improved compared with those previously reported.

SUBMITTER: Watanabe N 

PROVIDER: S-EPMC11270884 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature