The astrovirus N-terminal nonstructural protein anchors replication complexes to the perinuclear ER membranes.

ABSTRACT: An essential aspect of positive-sense RNA virus replication is anchoring the replication complex (RC) to cellular membranes. Positive-sense RNA viruses employ diverse strategies, including co-translational membrane targeting through signal peptides and co-opting cellular membrane trafficking components. Often, N-terminal nonstructural proteins play a crucial role in linking the RC to membranes, facilitating the early association of the replication machinery. Astroviruses utilize a polyprotein strategy to synthesize nonstructural proteins, relying on subsequent processing to form replication-competent complexes. This study provides evidence for the perinuclear ER membrane association of RCs in five distinct human astrovirus strains. Using tagged recombinant classical human astrovirus 1 and

SUBMITTER: Ali H 

PROVIDER: S-EPMC11271882 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature