One-pot Golden Gate Assembly of an avian infectious bronchitis virus reverse genetics system.

ABSTRACT: Avian infectious bronchitis is an acute respiratory disease of poultry of particular concern for global food security. Investigation of infectious bronchitis virus (IBV), the causative agent of avian infectious bronchitis, via reverse genetics enables deeper understanding of virus biology and a rapid response to emerging variants. Classic methods of reverse genetics for IBV can be time consuming, rely on recombination for the introduction of mutations, and, depending on the system, can be subject to genome instability and unreliable success rates. In this study, we have applied data-optimized Golden Gate Assembly design to create a rapidly executable, flexible, and faithful reverse genetics system for IBV. The IBV genome was divided into 12 fragments at high-fidelity fusion site breakpoint

SUBMITTER: Bilotti K 

PROVIDER: S-EPMC11271894 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature