Unveiling diabetic nephropathy: a novel diagnostic model through single-cell sequencing and co-expression analysis.

ABSTRACT:

Background

Diabetic nephropathy (DN) is a severe complication of diabetes that affects the kidneys. Disulfidptosis, a newly defined type of programmed cell death, has emerged as a potential area of interest, yet its significance in DN remains unexplored.

Methods

This study utilized single-cell sequencing data GSE131882 from GEO database combined with bulk transcriptome sequencing data GSE30122, GSE30528 and GSE30529 to investigate disulfidptosis in DN. Single-cell sequencing analysis was performed on samples from DN patients and healthy controls, focusing on cell heterogeneity and communication. Weighted gene co-expression network analysis (WGCNA) and gene set enrichment analysis (GSEA) were employed to identify disulfidptosis-related gene sets and pathways. A diagnostic mod

SUBMITTER: Wang G 

PROVIDER: S-EPMC11272118 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature