Temporally resolved early bone morphogenetic protein-driven transcriptional cascade during human amnion specification.

ABSTRACT: Amniogenesis, a process critical for continuation of healthy pregnancy, is triggered in a collection of pluripotent epiblast cells as the human embryo implants. Previous studies have established that bone morphogenetic protein (BMP) signaling is a major driver of this lineage specifying process, but the downstream BMP-dependent transcriptional networks that lead to successful amniogenesis remain to be identified. This is, in part, due to the current lack of a robust and reproducible model system that enables mechanistic investigations exclusively into amniogenesis. Here, we developed an improved model of early amnion specification, using a human pluripotent stem cell-based platform in which the activation of BMP signaling is controlled and synchronous. Uniform amniogenesis is seen within 4

SUBMITTER: Sekulovski N 

PROVIDER: S-EPMC11272160 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature