Multianalytical Approach to Understand Polyphenol-Mal d 1 Interactions to Predict Their Impact on the Allergenic Potential of Apples.

ABSTRACT: Interactions between phenolic compounds and the allergen Mal d 1 are discussed to be the reason for better tolerance of apple cultivars, which are rich in polyphenols. Because Mal d 1 is susceptible to proteolytic digestion and allergenic symptoms are usually restricted to the mouth and throat area, the release of native Mal d 1 during the oral phase is of particular interest. Therefore, we studied the release of Mal d 1 under different in vitro oral digestion conditions and revealed that only 6-15% of the total Mal d 1 present in apples is released. To investigate proposed polyphenol-Mal d 1 interactions, various analytical methods, e.g., isothermal titration calorimetry, ¹H-¹⁵N-HSQC NMR, and untargeted mass spectrometry, were applied. For monomeric polyphenols, only

SUBMITTER: A H Kaeswurm J 

PROVIDER: S-EPMC11273618 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature