Sanguinarine Induces Necroptosis of HCC by Targeting PKM2 Mediated Energy Metabolism.

ABSTRACT:

Backgrounds

Abnormal metabolism is the hallmark of hepatocellular carcinoma. Targeting energy metabolism has become the major focus of cancer therapy. The natural product, sanguinarine, displays remarkable anti-tumor properties by disturbing energy homeostasis; however, the underlying mechanism has not yet been elucidated.

Methods

The anticancer activity of sanguinarine was determined using CCK-8 and colony formation assay. Morphological changes of induced cell death were observed under electron microscopy. Necroptosis and apoptosis related markers were detected using western blotting. PKM2 was identified as the target by transcriptome sequencing. Molecular docking assay was used to evaluate the binding affinity of sanguinarine to the PKM2 molecule. Furthermore, Alb-Cre

SUBMITTER: Kong R 

PROVIDER: S-EPMC11274805 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature