Electrochemical and Fluorescence MnO₂-Polymer Dot Electrode Sensor for Osteoarthritis-Based Peroxisomal β-Oxidation Knockout Model.

ABSTRACT: A coenzyme A (CoA-SH)-responsive dual electrochemical and fluorescence-based sensor was designed utilizing an MnO₂-immobilized-polymer-dot (MnO₂@D-PD)-coated electrode for the sensitive detection of osteoarthritis (OA) in a peroxisomal β-oxidation knockout model. The CoA-SH-responsive MnO₂@D-PD-coated electrode interacted sensitively with CoA-SH in OA chondrocytes, triggering electroconductivity and fluorescence changes due to cleavage of the MnO₂ nanosheet on the electrode. The MnO₂@D-PD-coated electrode can detect CoA-SH in immature articular chondrocyte primary cells, as indicated by the significant increase in resistance in the control medium (R_24h = 2.17 MΩ). This sensor also sensitively monitored the increase in resist

SUBMITTER: Robby AI 

PROVIDER: S-EPMC11275033 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature