Identifying essential genes in <i>Schaalia odontolytica</i> using a highly-saturated transposon library.

ABSTRACT: The unique epibiotic-parasitic relationship between Nanosynbacter lyticus type strain TM7x, a member of the newly identified Candidate Phyla Radiation, now referred to as Patescibacteria, and its basibiont, Schaalia odontolytica strain XH001 (formerly Actinomyces odontolyticus), require more powerful genetic tools for deeper understanding of the genetic underpinnings that mediate their obligate relationship. Previous studies have mainly characterized the genomic landscape of XH001 during or post TM7x infection through comparative genomic or transcriptomic analyses followed by phenotypic analysis. Comprehensive genetic dissection of the pair is currently cumbersome due to the lack of robust genetic tools in TM7x. However, basic genetic tools are available for XH0

SUBMITTER: Bedree JK 

PROVIDER: S-EPMC11275721 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature