Establishing a Male-Positive Genetic Sexing Strain in the Asian Malaria Vector Anopheles stephensi.

ABSTRACT: Genetic biocontrol interventions targeting mosquito-borne diseases require the release of male mosquitoes exclusively, as only females consume blood and transmit human pathogens. This reduces the risk of spreading pathogens while enabling effective population control. Robust sex sorting methods to enable early larval sorting in mosquitoes need to be developed to allow for scalable sex sorting for genetic biocontrol interventions. This study applies the SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter) system, previously developed for Aedes aegypti, to the Asian malaria vector Anopheles stephensi. We hypothesized that the intron from the doublesex gene in Anopheles gambiae would function in An. stephensi du

SUBMITTER: Weng SC 

PROVIDER: S-EPMC11275880 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature