Insights into CLC-0's Slow-Gating from Intracellular Proton Inhibition.

ABSTRACT: The opening of the Torpedo CLC-0 chloride (Cl^-) channel is known to be regulated by two gating mechanisms: fast gating and slow (common) gating. The structural basis underlying the fast-gating mechanism is better understood than that of the slow-gating mechanism, which is still largely a mystery. Our previous study on the intracellular proton (H⁺_i)-induced inhibition of the CLC-0 anionic current led to the conclusion that the inhibition results from the slow-gate closure (also called inactivation). The conclusion was made based on substantial evidence such as a large temperature dependence of the H⁺_i inhibition similar to that of the channel inactivation, a resistance to the H⁺_i inhibition in the inactivati

SUBMITTER: Kwon HC 

PROVIDER: S-EPMC11276645 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature