Metabolic Shift in Porcine Spermatozoa during Sperm Capacitation-Induced Zinc Flux.

ABSTRACT: Mammalian spermatozoa rely on glycolysis and mitochondrial oxidative phosphorylation for energy leading up to fertilization. Sperm capacitation involves a series of well-regulated biochemical steps that are necessary to give spermatozoa the ability to fertilize the oocyte. Additionally, zinc ion (Zn²⁺) fluxes have recently been shown to occur during mammalian sperm capacitation. Semen from seven commercial boars was collected and analyzed using image-based flow cytometry before, after, and with the inclusion of 2 mM Zn²⁺ containing in vitro capacitation (IVC) media. Metabolites were extracted and analyzed via Gas Chromatography-Mass Spectrometry (GC-MS), identifying 175 metabolites, with 79 differentially abundant across treatments (p < 0.05). Non-capacitated s

SUBMITTER: Weide T 

PROVIDER: S-EPMC11276750 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature