Using diffusion tensor imaging to depict myocardial changes after matured pluripotent stem cell-derived cardiomyocyte transplantation.

ABSTRACT:

Background

Novel treatment strategies are needed to improve the structure and function of the myocardium post-infarction. In vitro-matured pluripotent stem cell-derived cardiomyocytes (PSC-CMs) have been shown to be a promising regenerative strategy. We hypothesized that mature PSC-CMs will have anisotropic structure and improved cell alignment when compared to immature PSC-CMs using cardiovascular magnetic resonance (CMR) in a guinea pig model of cardiac injury.

Methods

Guinea pigs (n = 16) were cryoinjured on day -10, followed by transplantation of either 10⁸ polydimethylsiloxane (PDMS)-matured PSC-CMs (n = 6) or 10⁸ immature tissue culture plastic (TCP)-generated PSC-CMs (n = 6) on day 0. Vehicle (sham-treated) subjects were injected with a pro-survi

SUBMITTER: Cook MP 

PROVIDER: S-EPMC11278291 | biostudies-literature | 2024 Winter

REPOSITORIES: biostudies-literature