Implementation of Spore Display in Paenibacillus polymyxa with Different Hydrolytic Enzymes.

ABSTRACT: Biotechnological processes are essential for producing climate-friendly high-value chemicals or pharmaceutical compounds, which can include steps catalyzed by enzymes. Therefore, establishing new, robust, and cheap enzyme production processes is desirable. One possible way to enhance processes is through the use of the spore display method. Spore display can present heterologous proteins on the surface of bacterial spores, offering numerous advantages in a range of biotechnological applications. This study demonstrates the implementation of the spore display method in Paenibacillus polymyxa, achieved by modifying the spore surface, incorporating an anchoring protein, and attaching green fluorescent protein to it, allowing the visualization of fluorescent spores. Following the initia

SUBMITTER: Zander M 

PROVIDER: S-EPMC11278568 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature