A Photonic Immunosensor Detection Method for Viable and Non-Viable <i>E. coli</i> in Water Samples.

ABSTRACT: Detection and enumeration of coliform bacteria using traditional methods and current molecular techniques against E. coli usually involve long processes with less sensitivity and specificity to distinguish between viable and non-viable bacteria for microbiological water analysis. This approach involves developing and validating an immunosensor comprising ring resonators functionalized with specific antibodies surrounded by a network of microchannels as an alternative method for detecting and indirectly enumerating Escherichia coli in samples of water for consumption. Different ELISA assays were conducted to characterize monoclonal and polyclonal antibodies selected as detection probes for specific B-galactosidase enzymes and membrane LPS antigens of E. coli. An immobil

SUBMITTER: Fernandez Blanco A 

PROVIDER: S-EPMC11278787 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature