Semi-quantitative analysis of formaldehyde in food using calibration chart based on number of colored wells of microwell plate titration.

ABSTRACT: Microplate titration quantifies sodium hydroxide generated from formaldehyde reacting with excess sulfite in a 96-microwell plate. Phenolphthalein indicators change from red to colorless when all hydroxide ions react. Methodology optimized reagent concentrations, and reaction time and created a Calibration Chart for semi-quantitative determination. The chart shows formaldehyde concentration ranges corresponding to red well counts from 0 to 200 mM in 20 mM increments. Inter-operator repeatability demonstrates precision (3 replicates), correlating red wells with standard formaldehyde concentrations. This instrument-free technique uses readily available commercial plates, eliminating the need for specialized equipment and calibration. The methodology offers simplicity with its reliance on rea

SUBMITTER: Tongdee M 

PROVIDER: S-EPMC11280023 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature