Evaluation of Primers OPF-01, P54, and 1253 to Identify <i>A. fumigatus</i>, <i>A. flavus</i>, and <i>A. niger</i> from Polymorphic Patterns Obtained by RAPD-PCR.

ABSTRACT: We evaluated the specificity of the primers OPF-01, P54, and 1253 to identify A. fumigatus, A. flavus, and A. niger, respectively, with the RAPD-PCR method. Eighty-two isolates belonging to the sections Fumigati, Flavi, and Nigri were used. The isolates were identified by phenotypic (macro- and micromorphology) and genotypic (partial sequences of the BenA gene) methods. The RAPD-PCR method was used to obtain polymorphic patterns with the primers OPF-01, P54, and 1253. The specificity of the polymorphic patterns of the isolates of each species was evaluated through the UPGMA clustering method and logistic regression model. All isolates of the genus Aspergillus were identified at the section level by macro- and micromorphology showing t

SUBMITTER: Castro-Fuentes CA 

PROVIDER: S-EPMC11280055 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature