Excreted/Secreted 15-kDa Proteins and Serine Protease Peptides from <i>Haemonchus contortus</i> Act as Immune-Response Enhancers in Lambs.

ABSTRACT: This study assessed the immunoprotective effect in lambs of a native excreted/secreted 15-kDa protein and two synthesised S28 peptides derived from the infective transitory larvae (xL₃) and adult stages (AS) of Haemonchus contortus. Twenty-two Pelibuey lambs were divided into negative and positive control groups, as well as immunised lamb groups, with 100 µg of the 15-kDa native protein (15kDaNP) and S28 peptides (S28P). The eggs per gram (EPG) and haematocrit were measured, and AS were counted and morphologically measured. To assess the immunoprotection in lambs, indirect enzyme-linked immunosorbent assays and relative expression analyses of immune cytokines were performed using serum and abomasal samples. Our results showed a 72.28% reduction in adult worms (AW) in the

SUBMITTER: Camas-Pereyra R 

PROVIDER: S-EPMC11280362 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature