A Live Attenuated H1N1 Influenza Vaccine Based on the Mutated M Gene.

ABSTRACT: The influenza vaccines currently approved for clinical use mainly include inactivated influenza virus vaccines and live attenuated influenza vaccines (LAIVs). LAIVs have multiple advantages, such as ease of use and strong immunogenicity, and can provide cross-protection. In this study, the M gene of the PR8 virus was mutated as follows (G11T, C79G, G82C, C85G, and C1016A), and a live attenuated influenza virus containing the mutated M gene was rescued and obtained using reverse genetic technology as a vaccine candidate. The replication ability of the rescued virus was significantly weakened in both MDCK cells and mice with attenuated virulence. Studies on immunogenicity found that 1000 TCID₅₀ of mutated PR8 (mPR8) can prime strong humoral and cellular immune responses. Single-do

SUBMITTER: Yi Y 

PROVIDER: S-EPMC11281364 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature