The Structure of <i>Spiroplasma Virus 4</i>: Exploring the Capsid Diversity of the <i>Microviridae</i>.

ABSTRACT: Spiroplasma virus 4 (SpV4) is a bacteriophage of the Microviridae, which packages circular ssDNA within non-enveloped T = 1 icosahedral capsids. It infects spiroplasmas, which are known pathogens of honeybees. Here, the structure of the SpV4 virion is determined using cryo-electron microscopy to a resolution of 2.5 Å. A striking feature of the SpV4 capsid is the mushroom-like protrusions at the 3-fold axes, which is common among all members of the subfamily Gokushovirinae. While the function of the protrusion is currently unknown, this feature varies widely in this subfamily and is therefore possibly an adaptation for host recognition. Furthermore, on the interior of the SpV4 capsid, the location of DNA-binding protein VP8 was identified and shown to have low structura

SUBMITTER: Mietzsch M 

PROVIDER: S-EPMC11281519 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature