Designing a Conserved Immunogenic Peptide Construct from the Nucleocapsid Protein of <i>Puumala orthohantavirus</i>.

ABSTRACT: Puumala orthohantavirus (PUUV) is an emerging zoonotic virus endemic to Europe and Russia that causes nephropathia epidemica, a mild form of hemorrhagic fever with renal syndrome (HFRS). There are limited options for treatment and diagnosis of orthohantavirus infection, making the search for potential immunogenic candidates crucial. In the present work, various bioinformatics tools were employed to design conserved immunogenic peptides containing multiple epitopes of PUUV nucleocapsid protein. Eleven conserved peptides (90% conservancy) of the PUUV nucleocapsid protein were identified. Three conserved peptides containing multiple T and B cell epitopes were selected using a consensus epitope prediction algorithm. Molecular docking using the HPEP dock server demonstrated strong bindin

SUBMITTER: Sehgal A 

PROVIDER: S-EPMC11281540 | biostudies-literature | 2024 Jun

REPOSITORIES: biostudies-literature