Low-Input, High-Resolution 5' Terminal Filovirus RNA Sequencing with ViBE-Seq.

ABSTRACT: Although next-generation sequencing (NGS) has been instrumental in determining the genomic sequences of emerging RNA viruses, de novo sequence determination often lacks sufficient coverage of the 5' and 3' ends of the viral genomes. Since the genome ends of RNA viruses contain the transcription and genome replication promoters that are essential for viral propagation, a lack of terminal sequence information hinders the efforts to study the replication and transcription mechanisms of emerging and re-emerging viruses. To circumvent this, we have developed a novel method termed ViBE-Seq (Viral Bona Fide End Sequencing) for the high-resolution sequencing of filoviral genome ends using a simple yet robust protocol with high fidelity. This technique allows for sequence determination of the 5' en

SUBMITTER: Ross SJ 

PROVIDER: S-EPMC11281615 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature