Saccharomyces cerevisiae β-glucan improves the response of trained macrophages to severe P. aeruginosa infections.

ABSTRACT: OBJECTIVE P. AERUGINOSA: (PA), the major pathogen of lung cystic fibrosis (CF), polarizes macrophages into hyperinflammatory tissue damaging phenotype. The main aim of this study was to verify whether training of macrophages with β-glucan might improve their response to P. aeruginosa infections.

Methods

To perform this task C57BL/6 mice sensitive to infections with P. aeruginosa were used. Peritoneal macrophages were trained with Saccharomyces cerevisiae β-glucan and exposed to PA57, the strong biofilm-forming bacterial strain isolated from the patient with severe lung CF. The release of cytokines and the expression of macrophage phenotypic markers were measured. A quantitative proteomic approach was used for the characterization of proteome-wide changes in macrophages. The effect o

SUBMITTER: Ciszek-Lenda M 

PROVIDER: S-EPMC11282130 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature