Development of a defined medium for the heterotrophic cultivation of Metallosphaera sedula.

ABSTRACT: The heterotrophic cultivation of extremophilic archaea still heavily relies on complex media. However, complex media are associated with unknown composition, high batch-to-batch variability, potential inhibiting and interfering components, as well as regulatory challenges, hampering advancements of extremophilic archaea in genetic engineering and bioprocessing. For Metallosphaera sedula, a widely studied organism for biomining and bioremediation and a potential production host for archaeal ether lipids, efforts to find defined cultivation conditions have still been unsuccessful. This study describes the development of a novel chemically defined growth medium for M. sedula. Initial experiments with commonly used complex casein-derived media sources deciphered Casamino Acids as the most suit

SUBMITTER: Sedlmayr VL 

PROVIDER: S-EPMC11282131 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature