Sex-specific DNA-replication in the early mammalian embryo.

ABSTRACT: The timing of DNA replication in mammals is crucial for minimizing errors and influenced by genome usage and chromatin states. Replication timing in the newly formed mammalian embryo remains poorly understood. Here, we have investigated replication timing in mouse zygotes and 2-cell embryos, revealing that zygotes lack a conventional replication timing program, which then emerges in 2-cell embryos. This program differs from embryonic stem cells and generally correlates with transcription and genome compartmentalization of both parental genomes. However, consistent and systematic differences existed between the replication timing of the two parental genomes, including considerably later replication of maternal pericentromeric regions compared to paternal counterparts. Moreover, maternal chr

SUBMITTER: Halliwell JA 

PROVIDER: S-EPMC11282264 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature