Genome-wide association studies with experimental validation identify a protective role for B lymphocytes against chronic post-surgical pain.

ABSTRACT:

Background

Chronic post-surgical pain (CPSP) significantly impacts patients' recovery and quality of life. Although environmental risk factors are well-established, genetic risk remains less understood.

Methods

A meta-analysis of genome-wide association studies followed by partitioned heritability was performed on 1350 individuals across five surgery types: hysterectomy, mastectomy, abdominal, hernia, and knee. In subsequent animal studies, withdrawal thresholds to evoked mechanical stimulation were measured in Rag1 null mutant and wild-type mice after plantar incision and laparotomy. Cell sorting by flow cytometry tracked recruitment of immune cell types.

Results

We discovered 77 genome-wide significant single-nucleotide polymorphism (SNP) hits, distributed among 24

SUBMITTER: Parisien M 

PROVIDER: S-EPMC11282472 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature