Novel hypoxia-induced HIF-1αactivation in asthma pathogenesis.

ABSTRACT:

Background

Asthma's complexity, marked by airway inflammation and remodeling, is influenced by hypoxic conditions. This study focuses on the role of Hypoxia-Inducible Factor-1 Alpha (HIF-1α) and P53 ubiquitination in asthma exacerbation.

Methods

High-throughput sequencing and bioinformatics were used to identify genes associated with asthma progression, with an emphasis on GO and KEGG pathway analyses. An asthma mouse model was developed, and airway smooth muscle cells (ASMCs) were isolated to create an in vitro hypoxia model. Cell viability, proliferation, migration, and apoptosis were assessed, along with ELISA and Hematoxylin and Eosin (H&E) staining.

Results

A notable increase in HIF-1α was observed in both in vivo and in vitro asthma models. HIF-1α upregulation e

SUBMITTER: Wan M 

PROVIDER: S-EPMC11282634 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature