Development and validation of Mayaro virus with luciferase reporter genes as a tool for antiviral assays.

ABSTRACT: Arboviruses are etiological agents in an extensive group of emerging diseases with great clinical relevance in Brazil, due to the wide distribution of their vectors and the favorable environmental conditions. Among them, the Mayaro virus (MAYV) has drawn attention since its emergence as the etiologic agent of Mayaro fever, a highly debilitating disease. To study viral replication and identify new drug candidates, traditional antiviral assays based on viral antigens and/or plaque assays have been demonstrating low throughput, making it difficult to carry out larger-scale assays. Therefore, we developed and characterized two DNA-launched infectious clones reporter viruses based on the MAYV strain BeAr 20290 containing the reporter genes of firefly luciferase (FLuc) and nanolucifera

SUBMITTER: Marinho MDS 

PROVIDER: S-EPMC11283106 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature