Dataset Information

High-resolution plasma metabolomics and thiamine status in critically Ill adult patients.

ABSTRACT:

Introduction

Thiamine (Vitamin B1) is an essential micronutrient and is classically considered a co-factor in energy metabolism. The association between thiamine status and whole-body metabolism in critical illness has not been studied.

Objectives

To determine association between whole blood thiamine pyrophosphate (TPP) concentrations and plasma metabolites and connected metabolic pathways using high resolution metabolomics (HRM) in critically ill patients.

Methods

Cross-sectional study performed at Erciyes University Hospital, Kayseri, Turkey and Emory University, Atlanta, GA, USA. Participants were critically ill adults with an expected length of intensive care unit stay longer than 48 h and receiving chronic furosemide therapy. A total of 76 participants were included. Mean age was 69 years (range 33-92 years); 65% were female. Blood for TPP and metabolomics was obtained on the day of ICU admission. Whole blood TPP was measured by HPLC and plasma HRM was performed using liquid chromatography/mass spectrometry. Data was analyzed using regression analysis of TPP levels against all plasma metabolomic features in metabolome-wide association studies (MWAS). MWAS using the highest and lowest TPP concentration tertiles was performed as a secondary analysis.

Results

Specific metabolic pathways associated with whole blood TPP levels in regression and tertile analysis included pentose phosphate, fructose and mannose, branched chain amino acid, arginine and proline, linoleate, and butanoate pathways.

Conclusions

Plasma HRM revealed that thiamine status, determined by whole blood TPP concentrations, was significantly associated with metabolites and metabolic pathways related to metabolism of energy, carbohydrates, amino acids, lipids, and the gut microbiome in adult critically ill patients.

SUBMITTER: Gundogan K

PROVIDER: S-EPMC11283406 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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Publications

High-resolution plasma metabolomics and thiamine status in critically Ill adult patients.

Gundogan Kursat K Nellis Mary M MM Ozer Nurhayat T NT Ergul Serap S SS Sahin Gulsah G GG Temel Sahin S Yuksel Recep C RC Teeny Sami S Alvarez Jessica A JA Sungur Murat M Jones Dean P DP Ziegler Thomas R TR

Metabolomics : Official journal of the Metabolomic Society 20240727 4

<h4>Introduction</h4>Thiamine (Vitamin B1) is an essential micronutrient and is classically considered a co-factor in energy metabolism. The association between thiamine status and whole-body metabolism in critical illness has not been studied.<h4>Objectives</h4>To determine association between whole blood thiamine pyrophosphate (TPP) concentrations and plasma metabolites and connected metabolic pathways using high resolution metabolomics (HRM) in critically ill patients.<h4>Methods</h4>Cross-se ...[more]

PMID: 39066851

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Dataset Information

High-resolution plasma metabolomics and thiamine status in critically Ill adult patients.

Introduction

Objectives

Methods

Results

Conclusions

Publications

High-resolution plasma metabolomics and thiamine status in critically Ill adult patients.

Similar Datasets

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