Large-scale single-virus genomics uncovers hidden diversity of river water viruses and diversified gene profiles.

ABSTRACT: Environmental viruses (primarily bacteriophages) are widely recognized as playing an important role in ecosystem homeostasis through the infection of host cells. However, the majority of environmental viruses are still unknown as their mosaic structure and frequent mutations in their sequences hinder genome construction in current metagenomics. To enable the large-scale acquisition of environmental viral genomes, we developed a new single-viral genome sequencing platform with microfluidic-generated gel beads. Amplification of individual DNA viral genomes in mass-produced gel beads allows high-throughput genome sequencing compared to conventional single-virus genomics. The sequencing analysis of river water samples yielded 1431 diverse viral single-amplified genomes, whereas viral metagenom

SUBMITTER: Nishikawa Y 

PROVIDER: S-EPMC11283719 | biostudies-literature | 2024 Jan

REPOSITORIES: biostudies-literature