Designing monomeric IFNγ: The significance of domain-swapped dimer structure in IFNγ immune responses.

ABSTRACT: Interferon (IFN) γ can initiate immune responses by inducing the expression of major histocompatibility complex molecules, suggesting its potential for cancer immunotherapy. However, it also has an immunosuppressive function that limits its application as a therapeutic agent. IFNγ has a characteristic domain-swapped dimer structure with two of the six α-helices exchanged with each other. As we hypothesized that the contrasting functions of IFNγ could be attributed to its unique domain-swapped structure, we designed monomeric IFNγ by transforming the domain-swapped dimer structure of WT IFNγ. We conjectured the evolution of this domain-swapped dimer and hypothesized that the current IFNγ structure emerged through shortening of the loop structure at the base of the swapped domain and the acc

SUBMITTER: Goto Y 

PROVIDER: S-EPMC11284706 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature