Stable and reusable calcium-responsive biopolymer for affinity precipitation of therapeutic antibodies.

ABSTRACT: Affinity precipitation is an attractive method for protein purification due to its many advantages, including the rapid capture of target proteins, simple processing, high specificity, and ease of scale-up. We previously reported a robust antibody purification method using Ca²⁺-dependent precipitation of ZZ-hCSQ2, a fusion protein of human calsequestrin 2, and the antibody-binding protein ZZ. However, the stability of this fusion protein was not sufficiently high for industrial use because the antibody recovery yield decreased to 60% after being reused 10 times. To identify a more stable calsequestrin (CSQ), we calculated Rosetta energy values for the folding stabilities of various CSQ homologs and selected human CSQ1 (hCSQ1) with lowest energy value (-992.6) as the new CSQ plat

SUBMITTER: Park H 

PROVIDER: S-EPMC11285868 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature