Immunological characteristics of a recombinant alphaherpesvirus with an envelope-embedded <i>Cap</i> protein of circovirus.

ABSTRACT:

Introduction

Variant pseudorabies virus (PRV) is a newly emerged zoonotic pathogen that can cause human blindness. PRV can take advantage of its large genome and multiple non-essential genes to construct recombinant attenuated vaccines carrying foreign genes. However, a major problem is that the foreign genes in recombinant PRV are only integrated into the genome for independent expression, rather than assembled on the surface of virion.

Methods

We reported a recombinant PRV with deleted gE/TK genes and an inserted porcine circovirus virus 2 (PCV2) Cap gene into the extracellular domain of the PRV gE gene using the Cre-loxP recombinant system combined with the CRISPR-Cas9 gene editing system. This recombinant PRV (PRV-Cap), with the envelope-embedded Cap protein, exhi

SUBMITTER: Lu C 

PROVIDER: S-EPMC11286414 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature