The small CRL4^CSA ubiquitin ligase component DDA1 regulates transcription-coupled repair dynamics.

ABSTRACT: Transcription-blocking DNA lesions are specifically targeted by transcription-coupled nucleotide excision repair (TC-NER), which removes a broad spectrum of DNA lesions to preserve transcriptional output and thereby cellular homeostasis to counteract aging. TC-NER is initiated by the stalling of RNA polymerase II at DNA lesions, which triggers the assembly of the TC-NER-specific proteins CSA, CSB and UVSSA. CSA, a WD40-repeat containing protein, is the substrate receptor subunit of a cullin-RING ubiquitin ligase complex composed of DDB1, CUL4A/B and RBX1 (CRL4^CSA). Although ubiquitination of several TC-NER proteins by CRL4^CSA has been reported, it is still unknown how this complex is regulated. To unravel the dynamic molecular interactions and the regulation of this c

SUBMITTER: Llerena Schiffmacher DA 

PROVIDER: S-EPMC11286758 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature