High-throughput transcriptomics of 409 bacteria-drug pairs reveals drivers of gut microbiota perturbation.

ABSTRACT: Many drugs can perturb the gut microbiome, potentially leading to negative health consequences. However, mechanisms of most microorganism-drug responses have not been elucidated at the genetic level. Using high-throughput bacterial transcriptomics, we systematically characterized the gene expression profiles of prevalent human gut bacteria exposed to the most frequently prescribed orally administered pharmaceuticals. Across >400 drug-microorganism pairs, significant and reproducible transcriptional responses were observed, including pathways involved in multidrug resistance, metabolite transport, tartrate metabolism and riboflavin biosynthesis. Importantly, we discovered that statin-mediated upregulation of the AcrAB-TolC efflux pump in Bacteroidales species enhances microbial sensitivity

SUBMITTER: Ricaurte D 

PROVIDER: S-EPMC11287798 | biostudies-literature | 2024 Feb

REPOSITORIES: biostudies-literature