Detection and Quantification of 5moU RNA Modification from Direct RNA Sequencing Data.

ABSTRACT:

Background

Chemically modified therapeutic mRNAs have gained momentum recently. In addition to commonly used modifications (e.g., pseudouridine), 5moU is considered a promising substitution for uridine in therapeutic mRNAs. Accurate identification of 5-methoxyuridine (5moU) would be crucial for the study and quality control of relevant in vitro-transcribed (IVT) mRNAs. However, current methods exhibit deficiencies in providing quantitative methodologies for detecting such modification. Utilizing the capabilities of Oxford nanopore direct RNA sequencing, in this study, we present NanoML-5moU, a machine-learning framework designed specifically for the read-level detection and quantification of 5moU modification for IVT data.

Materials and methods

Nanopore direct

SUBMITTER: Li J 

PROVIDER: S-EPMC11288159 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature