Single-cell and spatial multiomic inference of gene regulatory networks using SCRIPro.

ABSTRACT:

Motivation

The burgeoning generation of single-cell or spatial multiomic data allows for the characterization of gene regulation networks (GRNs) at an unprecedented resolution. However, the accurate reconstruction of GRNs from sparse and noisy single-cell or spatial multiomic data remains challenging.

Results

Here, we present SCRIPro, a comprehensive computational framework that robustly infers GRNs for both single-cell and spatial multi-omics data. SCRIPro first improves sample coverage through a density clustering approach based on multiomic and spatial similarities. Additionally, SCRIPro scans transcriptional regulator (TR) importance by performing chromatin reconstruction and in silico deletion analyses using a comprehensive reference covering 1,292 human and 994 mouse T

SUBMITTER: Chang Z 

PROVIDER: S-EPMC11288411 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature