Macrophages modulate mesenchymal stem cell function via tumor necrosis factor alpha in tooth extraction model.

ABSTRACT: Mesenchymal stem cells (MSCs) and macrophages collaboratively contribute to bone regeneration after injury. However, detailed mechanisms underlying the interaction between MSCs and inflammatory macrophages (M1) remain unclear. A macrophage-depleted tooth extraction model was generated in 5-wk-old female C57BL/6J mice using clodronate liposome (12.5 mg/kg/mouse, intraperitoneally) or saline injection (control) before maxillary first molar extraction. Mice were sacrificed on days 1, 3, 5, 7, and 10 after tooth extraction (n = 4). Regenerated bone volume evaluation of tooth extraction socket (TES) and histochemical analysis of CD80⁺M1, CD206⁺M2 (anti-inflammatory macrophages), PDGFRα⁺MSC, and TNF-α⁺ cells were performed. In vitro, isolated MS

SUBMITTER: Mun AY 

PROVIDER: S-EPMC11289833 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature