High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.

ABSTRACT: Vitrification is the most promising method for cryopreservation of complex structures such as organs and tissue constructs. However, this method requires multimolar concentrations of cell-permeant cryoprotective agents (CPAs), which can be toxic at such elevated levels. The selection of CPAs for organ vitrification has been limited to a few chemicals; however, there are numerous chemicals with properties similar to commonly used CPAs. In this study, we developed a high-throughput method that significantly increases the speed of cell membrane permeability measurement, enabling ~100 times faster permeability measurement than previous methods. The method also allows assessment of CPA toxicity using the same 96-well plate. We tested five commonly used CPAs and 22 less common ones at both 4 °C

SUBMITTER: Ahmadkhani N 

PROVIDER: S-EPMC11291039 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature