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Nanoscale organization is changed in native, surface AMPARs by mouse brain region and tauopathy.

ABSTRACT: Synaptic AMPA receptors (AMPARs) on neuronal plasma membranes are correlated with learning and memory. Using a unique labeling and super-resolution imaging, we have visualized the nanoscale synaptic and extra-synaptic organization of native surface AMPARs for the first time in mouse brain slices as a function of brain region and tauopathy. We find that the fraction of surface AMPARs organized in synaptic clusters is two-times smaller in the hippocampus compared to the motor and somatosensory cortex. In 6 months old PS19 model of tauopathy, synaptic and extrasynaptic distributions are disrupted in the hippocampus but not in the cortex. Thus, this optimized super-resolution imaging tool allows us to observe synaptic deterioration at the onset of tauopathy before apparent neurodegeneration.

SUBMITTER: Vaidya RM

PROVIDER: S-EPMC11291066 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature

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Nanoscale organization is changed in native, surface AMPARs by mouse brain region and tauopathy.

Vaidya Rohit M RM Zhang Jiahao J Nall Duncan D Lee Yongjae Y Chang Kim Eung E Ma Donghan D Huang Fang F Nonaka Hiroshi H Kiyonaka Shigeki S Hamachi Itaru I Jung Chung Hee H Selvin Paul R PR

bioRxiv : the preprint server for biology 20240723

Synaptic AMPA receptors (AMPARs) on neuronal plasma membranes are correlated with learning and memory. Using a unique labeling and super-resolution imaging, we have visualized the nanoscale synaptic and extra-synaptic organization of native <i>surface</i> AMPARs for the first time in mouse brain slices as a function of brain region and tauopathy. We find that the fraction of surface AMPARs organized in synaptic clusters is two-times smaller in the hippocampus compared to the motor and somatosens ...[more]

PMID: 39091751

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