Microbiome-host interactions in the pathogenesis of acute exacerbation of chronic obstructive pulmonary disease.

ABSTRACT:

Objective

To explore the underlying mechanisms the airway microbiome contributes to Acute Exacerbation of Chronic Obstructive Pulmonary Disease(AECOPD).

Methods

We enrolled 31 AECOPD patients and 26 stable COPD patients, their sputum samples were collected for metagenomic and RNA sequencing, and then subjected to bioinformatic analyses. The expression of host genes was validated by Quantitative Real-time PCR(qPCR) using the same batch of specimens.

Results

Our results indicated a higher expression of Rothia mucilaginosa(p=0.015) in the AECOPD group and Haemophilus influenzae(p=0.005) in the COPD group. The Different expressed genes(DEGs) detected were significantly enriched in "type I interferon signaling pathway"(p<0.001, q=0

SUBMITTER: Li Y 

PROVIDER: S-EPMC11291260 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature