A humanized mouse that mounts mature class-switched, hypermutated and neutralizing antibody responses.

ABSTRACT: Humanized mice are limited in terms of modeling human immunity, particularly with regards to antibody responses. Here we constructed a humanized (THX) mouse by grafting non-γ-irradiated, genetically myeloablated Kit^W-41J mutant immunodeficient pups with human cord blood CD34⁺ cells, followed by 17β-estradiol conditioning to promote immune cell differentiation. THX mice reconstitute a human lymphoid and myeloid immune system, including marginal zone B cells, germinal center B cells, follicular helper T cells and neutrophils, and develop well-formed lymph nodes and intestinal lymphoid tissue, including Peyer's patches, and human thymic epithelial cells. These mice have diverse human B cell and T cell antigen receptor repertoires and can mount mature T cell-dependent and

SUBMITTER: Chupp DP 

PROVIDER: S-EPMC11291283 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature