Well Plate-Based Localized Electroporation Workflow for Rapid Optimization of Intracellular Delivery.

ABSTRACT: Efficient and nontoxic delivery of foreign cargo into cells is a critical step in many biological studies and cell engineering workflows with applications in areas such as biomanufacturing and cell-based therapeutics. However, effective molecular delivery into cells involves optimizing several experimental parameters. In the case of electroporation-based intracellular delivery, there is a need to optimize parameters like pulse voltage, duration, buffer type, and cargo concentration for each unique application. Here, we present the protocol for fabricating and utilizing a high-throughput multi-well localized electroporation device (LEPD) assisted by deep learning-based image analysis to enable rapid optimization of experimental parameters for efficient and nontoxic molecular delivery into c

SUBMITTER: Patino CA 

PROVIDER: S-EPMC11291937 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature