RTCB deficiency triggers colitis in mice by influencing the NF-κB and Wnt/β-catenin signaling pathways.

ABSTRACT: RNA terminal phosphorylase B (RTCB) has been shown to play a significant role in multiple physiological processes. However, the specific role of RTCB in the mouse colon remains unclear. In this study, we employ a conditional knockout mouse model to investigate the effects of RTCB depletion on the colon and the potential molecular mechanisms. We assess the efficiency and phenotype of Rtcb knockout using PCR, western blot analysis, histological staining, and immunohistochemistry. Compared with the control mice, the Rtcb-knockout mice exhibit compromised colonic barrier integrity and prominent inflammatory cell infiltration. In the colonic tissues of Rtcb-knockout mice, the protein levels of TNF-α, IL-8, and p-p65 are increased, whereas the levels of IKKβ and IκBα are dec

SUBMITTER: Liu P 

PROVIDER: S-EPMC11292128 | biostudies-literature | 2024 Mar

REPOSITORIES: biostudies-literature