Enhancing clinical safety in bioengineered-root regeneration: The use of animal component-free medium.

ABSTRACT:

Background

Most studies used animal serum-containing medium for bioengineered-root regeneration, but ethical and safety issues raised by animal serum are a potentially significant risk for clinical use. Thus, this study aimed to find a safer method for bioengineered-root regeneration.

Methods

The biological properties of human dental pulp stem cells (hDPSCs) cultured in animal component-free (ACF) medium or serum-containing medium (5%, 10% serum-containing medium, SCM) were compared in vitro. hDPSCs were cultured in a three-dimensional (3D) environment with human-treated dentin matrix (hTDM). The capacity for odontogenesis was compared using quantitative real-time PCR (qPCR) and Western blot. Subsequently, the hDPSCs/hTDM complexes were transplanted into nude mice sub

SUBMITTER: Zhan Y 

PROVIDER: S-EPMC11292241 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature