In-vitro safety assessment of meropenem on human retinal pigment epithelium (RPE).

ABSTRACT:

Purpose

Endophthalmitis is a severe infection accompanied by inflammation that affects the anterior and posterior parts of the eye. It is typically treated with a combination of antibiotics that cover various microorganisms. However, retinal pigment epithelium (RPE) cells are highly susceptible to damage from intravitreal injection therapy. This study aimed to investigate the impact of clinically relevant concentrations of meropenem (alone or in combination with vancomycin) on the viability and inflammation of RPE cells.

Design

In-vitro Study.

Methods

RPE cells from passages 5-7 were treated with different concentrations of meropenem (1/4x, x, and 4x; [x = 16 mg/L]), vancomycin (30 mg/L), and meropenem (x) plus vancomycin for 24 h. The morphology assessment and MTT (3

SUBMITTER: Sanie-Jahromi F 

PROVIDER: S-EPMC11292269 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature