Transcriptome analyses of murine right and left maxilla-mandibular complex.

ABSTRACT:

Objective

The objective of the study was to investigate differential gene expression between murine right and left maxilla-mandibular (MxMn) complexes.

Setting and sample population

Wild-type (WT) C57BL/6 embryonic (E) day 14.5 (n = 3) and 18.5 (n = 3) murine embryos.

Methods

The E14.5 and 18.5 embryos were harvested and hemi-sectioned the MxMn complexes into right and left halves in the mid-sagittal plane. We isolated total RNA using Trizol reagent and further purified using the RNA-easy kit (QIAGEN). We confirmed equal expression of house-keeping genes in right and left halves using RT-PCR and then performed paired-end whole mRNA sequencing in LC Sciences (Houston, TX) followed by differential transcript analyses (>1 or <-1 log fold change; p < .05; q < .05; and FPK

SUBMITTER: Crawford J 

PROVIDER: S-EPMC11292856 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature