Sex hormones differently regulate lipid metabolism genes in primary human hepatocytes.

ABSTRACT: Prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD) is higher in men than in women. Hormonal and genetic causes may account for the sex differences in MASLD. Current human in vitro liver models do not sufficiently take the influence of biological sex and sex hormones into consideration. Primary human hepatocytes (PHHs) were isolated from liver specimen of female and male donors and cultured with sex hormones (17β-estradiol, testosterone and progesterone) for up to 72 h. mRNA expression levels of 8 hepatic lipid metabolism genes were analyzed by RT-qPCR. Sex hormones and their metabolites were determined in cell culture supernatants by LC-MS analyses. A sex-specific expression was observed for LDLR (low density lipoprotein receptor) with higher mRNA levels in male

SUBMITTER: Seidemann L 

PROVIDER: S-EPMC11292922 | biostudies-literature | 2024 Aug

REPOSITORIES: biostudies-literature