CRISPR-Cas9 knockout screen informs efficient reduction of the Komagataella phaffii secretome.

ABSTRACT:

Background

The yeast Komagataella phaffii is widely used for manufacturing recombinant proteins, but secreted titers of recombinant proteins could be improved by genetic engineering. In this study, we hypothesized that cellular resources could be redirected from production of endogenous proteins to production of recombinant proteins by deleting unneeded endogenous proteins. In non-model microorganisms such as K. phaffii, however, genetic engineering is limited by lack gene annotation and knowledge of gene essentiality.

Results

We identified a set of endogenous secreted proteins in K. phaffii by mass spectrometry and signal peptide prediction. Our efforts to disrupt these genes were hindered by limited annotation of essential genes. To predict essential genes, therefore, we d

SUBMITTER: Dalvie NC 

PROVIDER: S-EPMC11293167 | biostudies-literature | 2024 Jul

REPOSITORIES: biostudies-literature